NSJ Bioreagents

TRMT5 Antibody / tRNA methyltransferase 5

Product Code:
 
NSJ-RQ7568
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TRMT5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Immunofluorescent staining of FFPE human U-2 OS cells with TRMT5 antibody (red) and Tubulin Alpha mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 2
Western blot testing of human MCF7 cell lysate with TRMT5 antibody. Predicted molecular weight ~58 kDa, commonly observed at 50-60 kDa.
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Immunofluorescent staining of FFPE human U-2 OS cells with TRMT5 antibody (red) and Tubulin Alpha mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human MCF7 cell lysate with TRMT5 antibody. Predicted molecular weight ~58 kDa, commonly observed at 50-60 kDa.

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NSJ-RQ7568-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R291-E501) was used as the immunogen for the TRMT5 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q32P41