NSJ Bioreagents

C-terminal-binding protein 2 Antibody / CTBP2

Product Code:
 
NSJ-RQ7571
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
7F3E1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the C-terminal-binding protein 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
IHC staining of FFPE mouse colon tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE mouse rat tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human placental tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human colorectal adenocarcinoma tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE human thyroid cancer tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of 1) human HeLa, 2) human SiHa, 3) human COLO-320, 4) human SW620, 5) rat stomach, 6) rat brain, 7) mouse stomach and 8) mouse brain tissue lysate with C-terminal-binding protein 2 antibody. Predicted molecular weight ~49 kDa.
8 / 8
Flow cytometry testing of human U-87 MG cells with C-terminal-binding protein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= C-terminal-binding protein 2 antibody.
❮❯

IHC staining of FFPE mouse colon tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse rat tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human SiHa, 3) human COLO-320, 4) human SW620, 5) rat stomach, 6) rat brain, 7) mouse stomach and 8) mouse brain tissue lysate with C-terminal-binding protein 2 antibody. Predicted molecular weight ~49 kDa.
Flow cytometry testing of human U-87 MG cells with C-terminal-binding protein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= C-terminal-binding protein 2 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ7571-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The E1a region of group C adenoviruses encodes 2 nearly identical proteins that are largely responsible for the oncogenic properties of adenoviruses. The CTBP1 protein binds to the C-terminal half of these E1A proteins. It's predicted that CTBP2 is a 445-amino acid protein and it is 72% identical to CTBP1. The CTBP2 gene is mapped to chromosome 10q26.13. CTBP2 is a mammalian corepressor that targets diverse transcriptional regulators. It bounds the short medial portion of delta-EF1 containing the PLDLSL motif and it enhances transrepression activity of delta-EF1.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H321-Q445) was used as the immunogen for the C-terminal-binding protein 2 antibody.
Localisation:
Nucleus, Cell junction
Purity:
Antigen affinity purified
Uniprot #:
P56545