NSJ Bioreagents

Y-box-binding protein 1 Antibody / YB1 / MYS1

Product Code:
 
NSJ-RQ7576
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Y-box-binding protein 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of mouse ANA-1 cells with Y-box-binding protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Y-box-binding protein 1 antibody.
2 / 2
Western blot testing of 1) rat RH35, 2) mouse spleen, 3) mouse testis and 4) mouse Neuro-2a cell lysate with Y-box-binding protein 1 antibody. Expected molecular weight: 39~50 kDa depending on glycosylation level.

Flow cytometry testing of mouse ANA-1 cells with Y-box-binding protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Y-box-binding protein 1 antibody.
Western blot testing of 1) rat RH35, 2) mouse spleen, 3) mouse testis and 4) mouse Neuro-2a cell lysate with Y-box-binding protein 1 antibody. Expected molecular weight: 39~50 kDa depending on glycosylation level.

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CodeSizePrice
NSJ-RQ7576-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Y box binding protein 1, also known as Y-box transcription factor or nuclease-sensitive element-binding protein 1, is a protein that in humans is encoded by the YBX1 gene. Enables chromatin binding activity; mRNA binding activity; and single-stranded DNA binding activity. Involved in epidermis development and negative regulation of cellular senescence. Acts upstream of or within several processes, including cellular response to interleukin-7; negative regulation of striated muscle cell differentiation; and negative regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Part of histone pre-mRNA 3'end processing complex. Is expressed in several structures, including branchial arch; central nervous system; facial prominence; gonad; and retina. Orthologous to human YBX1 (Y-box binding protein 1).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RRFPPYYMRRPYARRPQYSNPPVQ from the human protein were used as the immunogen for the Y-box-binding protein 1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P62960