NSJ Bioreagents

TOM1L1 Antibody / TOM1-like protein 1

Product Code:
 
NSJ-RQ7577
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TOM1L1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 11
Immunofluorescent staining of FFPE human ovarian cancer tissue with TOM1L1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 11
Immunofluorescent staining of FFPE human rectal cancer tissue with TOM1L1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 11
IHC staining of FFPE human liver cancer tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human colon cancer tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human prostate adenocarcinoma tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human thyroid cancer tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human lung cancer tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE mouse brain tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
IHC staining of FFPE rat brain tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 11
Western blot testing of human 1) HeLa, 2) 293T, 3) T-47D and 4) HEL cell lysate with TOM1L1 antibody. Predicted molecular weight ~53 kDa.
11 / 11
Flow cytometry testing of human JK cells with TOM1L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOM1L1 antibody.
❮❯

Immunofluorescent staining of FFPE human ovarian cancer tissue with TOM1L1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human rectal cancer tissue with TOM1L1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human liver cancer tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with TOM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) 293T, 3) T-47D and 4) HEL cell lysate with TOM1L1 antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human JK cells with TOM1L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOM1L1 antibody.

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CodeSizePrice
NSJ-RQ7577-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
TOM1-like protein 1 is a protein that in humans is encoded by the TOM1L1 gene. Enables clathrin binding activity and protein kinase binding activity. Involved in negative regulation of mitotic nuclear division; positive regulation of protein phosphorylation; and signal transduction. Located in cytosol and endosome.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D9-D448) was used as the immunogen for the TOM1L1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
O75674