NSJ Bioreagents

TOMM34 Antibody / Transl°Case of outer membrane 34 kDa subunit

Product Code:
 
NSJ-RQ7578
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TOMM34 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 10
IHC staining of FFPE human lung cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 10
IHC staining of FFPE human liver cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human breast cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human cervical cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human prostate cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human colon cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human placental tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE rat testis tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 10
Western blot testing of 1) human A549, 2) human A375, 3) human HeLa, 4) human 293T, 5) rat testis and 6) mouse testis tissue lysate with TOMM34 antibody. Predicted molecular weight ~34 kDa.
10 / 10
Flow cytometry testing of human Daudi cells with TOMM34 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOMM34 antibody.

IHC staining of FFPE human lung cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis tissue with TOMM34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human A549, 2) human A375, 3) human HeLa, 4) human 293T, 5) rat testis and 6) mouse testis tissue lysate with TOMM34 antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of human Daudi cells with TOMM34 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOMM34 antibody.

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CodeSizePrice
NSJ-RQ7578-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Mitochondrial import receptor subunit TOM34 is a protein that in humans is encoded by the TOMM34 gene. The protein encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-H309) was used as the immunogen for the TOMM34 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q15785