NSJ Bioreagents

RRP7A Antibody / Ribosomal RNA-pr°Cessing protein 7 homolog A

Product Code:
 
NSJ-RQ7580
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RRP7A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human MCF7 cells with RRP7A antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human testicular seminoma tissue with RRP7A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human A431, 2) human MCF7, 3) human 293T and 4) monkey COS-7 cell lysate with RRP7A antibody. Expected molecular weight: 32-42 kDa.
4 / 4
Flow cytometry testing of human SiHa cells with RRP7A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RRP7A antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with RRP7A antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human testicular seminoma tissue with RRP7A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human A431, 2) human MCF7, 3) human 293T and 4) monkey COS-7 cell lysate with RRP7A antibody. Expected molecular weight: 32-42 kDa.
Flow cytometry testing of human SiHa cells with RRP7A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RRP7A antibody.

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CodeSizePrice
NSJ-RQ7580-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ribosomal RNA-processing protein 7 homolog A is a protein that in humans is encoded by the RRP7A gene. Enables RNA binding activity. Predicted to be involved in rRNA processing and ribosomal small subunit assembly. Predicted to act upstream of or within blastocyst formation. Predicted to be located in nucleoplasm. Predicted to be part of CURI complex and UTP-C complex. Implicated in primary autosomal recessive microcephaly.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-D181) was used as the immunogen for the RRP7A antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9Y3A4