NSJ Bioreagents

TRIM16 Antibody / Tripartite motif-containing protein 16

Product Code:
 
NSJ-RQ7582
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TRIM16 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 9
IHC staining of FFPE human colorectal adenocarcinoma tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 9
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human lung cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human liver cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human cervical cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human prostate cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
IHC staining of FFPE rat colon tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 9
Western blot testing of human 1) RT4 and 2) HeLa cell lysate with TRIM16 antibody. Predicted molecular weight: 54-63 kDa (multiple isoforms).
9 / 9
Flow cytometry testing of human Daudi cells with TRIM16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIM16 antibody.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) RT4 and 2) HeLa cell lysate with TRIM16 antibody. Predicted molecular weight: 54-63 kDa (multiple isoforms).
Flow cytometry testing of human Daudi cells with TRIM16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIM16 antibody.

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CodeSizePrice
NSJ-RQ7582-100ug100 ug£535.00
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Tripartite motif-containing protein 16 is a protein that in humans is encoded by the TRIM16 gene. The protein encoded by this gene is a tripartite motif (TRIM) family member that contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. While it lacks a RING domain found in other TRIM proteins, the encoded protein can homodimerize or heterodimerize with other TRIM proteins and has E3 ubiquitin ligase activity. This gene is also a tumor suppressor and is involved in secretory autophagy.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-Q218) was used as the immunogen for the TRIM16 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O95361