NSJ Bioreagents

SETD3 Antibody / Actin-histidine N-methyltransferase

Product Code:
 
NSJ-RQ7583
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SETD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 11
Western blot testing of 1) rat heart, 2) rat H9c2(2-1), 3) rat C6, 4) mouse heart, 5) mouse C2C12 and 6) mouse NIH 3T3 cell lysate with SETD3 antibody. Predicted molecular weight ~67 kDa.
2 / 11
Western blot testing of human 1) MCF7, 2) T-47D, 3) Jurkat, 4) MOLT4, 5) HL60, 6) PC-3 and 7) RT4 cell lysate with SETD3 antibody. Predicted molecular weight ~67 kDa.
3 / 11
IHC staining of FFPE human liver cancer tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human ovarian serous cancer tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human renal cancer tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human breast cancer tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human spleen tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
Immunofluorescent staining of FFPE human breast cancer tissue with SETD3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
9 / 11
Immunofluorescent staining of FFPE human U-2 OS cells with SETD3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 11
Flow cytometry testing of human 293T cells with SETD3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SETD3 antibody.
11 / 11
Flow cytometry testing of human HL60 cells with SETD3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SETD3 antibody.
❮❯

Western blot testing of 1) rat heart, 2) rat H9c2(2-1), 3) rat C6, 4) mouse heart, 5) mouse C2C12 and 6) mouse NIH 3T3 cell lysate with SETD3 antibody. Predicted molecular weight ~67 kDa.
Western blot testing of human 1) MCF7, 2) T-47D, 3) Jurkat, 4) MOLT4, 5) HL60, 6) PC-3 and 7) RT4 cell lysate with SETD3 antibody. Predicted molecular weight ~67 kDa.
IHC staining of FFPE human liver cancer tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous cancer tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SETD3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human breast cancer tissue with SETD3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with SETD3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human 293T cells with SETD3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SETD3 antibody.
Flow cytometry testing of human HL60 cells with SETD3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SETD3 antibody.

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CodeSizePrice
NSJ-RQ7583-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SET domain containing 3 (SETD3) is a protein that in humans is encoded by the SETD3 gene. SETD3 is a histone methyltransferase. In mouse muscle cells, Setd3 catalyzes mono- and dimethylation of histone H3 lysine-4 and H3K36 and promotes transcription of genes required for muscle cell differentiation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q173-R468) was used as the immunogen for the SETD3 antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q86TU7