NSJ Bioreagents

SGCE Antibody / Epsilon-sarcoglycan

Product Code:
 
NSJ-RQ7584
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SGCE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 9
IHC staining of FFPE human breast cancer tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 9
IHC staining of FFPE human liver cancer tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human renal cancer tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human spleen tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE mouse brain tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE rat brain tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
Western blot testing of 1) human U-251, 2) rat brain, 3) rat heart, 4) rat C6, 5) mouse brain and 6) mouse heart tissue lysate with SGCE antibody. Predicted molecular weight ~50 kDa.
8 / 9
Flow cytometry testing of human PC-3 cells with SGCE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SGCE antibody.
9 / 9
Flow cytometry testing of human A549 cells with SGCE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SGCE antibody.
❮❯

IHC staining of FFPE human breast cancer tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with SGCE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-251, 2) rat brain, 3) rat heart, 4) rat C6, 5) mouse brain and 6) mouse heart tissue lysate with SGCE antibody. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human PC-3 cells with SGCE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SGCE antibody.
Flow cytometry testing of human A549 cells with SGCE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SGCE antibody.

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CodeSizePrice
NSJ-RQ7584-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Epsilon-sarcoglycan is a protein that in humans is encoded by the SGCE gene. This gene encodes the epsilon member of the sarcoglycan family. Sarcoglycans are transmembrane proteins that are components of the dystrophin-glycoprotein complex, which link the actin cytoskeleton to the extracellular matrix. Unlike other family members which are predominantly expressed in striated muscle, the epsilon sarcoglycan is more broadly expressed. Mutations in this gene are associated with myoclonus-dystonia syndrome. This gene is imprinted, with preferential expression from the paternal allele. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A pseudogene associated with this gene is located on chromosome 2.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Y38-D407) was used as the immunogen for the SGCE antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O43556