NSJ Bioreagents

SEC31A Antibody

Product Code:
 
NSJ-RQ7589
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SEC31A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 15
IHC staining of FFPE human thyroid cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 15
IHC staining of FFPE human breast cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 15
IHC staining of FFPE human ovarian serous cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 15
IHC staining of FFPE human prostate adenocarcinoma cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 15
IHC staining of FFPE human renal cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 15
IHC staining of FFPE human spleen tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 15
IHC staining of FFPE mouse brain tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 15
IHC staining of FFPE mouse kidney tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 15
IHC staining of FFPE rat brain tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 15
IHC staining of FFPE rat kidney tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 15
IHC staining of FFPE rat colon tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 15
Immunofluorescent staining of FFPE human ovarian cancer tissue with SEC31A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
13 / 15
Immunofluorescent staining of FFPE human A549 cells with SEC31A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
14 / 15
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human K562, 4) human HepG2, 5) human U-87 MG, 6) rat PC-12 and 7) mouse NIH 3T3 cell lysate with SEC31A antibody. Predicted molecular weight : 107-133 kDa (multiple isoforms) with a possible ~57 kDa isoform.
15 / 15
Flow cytometry testing of human HL60 cells with SEC31A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC31A antibody.
❮❯

IHC staining of FFPE human thyroid cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with SEC31A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human ovarian cancer tissue with SEC31A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with SEC31A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human K562, 4) human HepG2, 5) human U-87 MG, 6) rat PC-12 and 7) mouse NIH 3T3 cell lysate with SEC31A antibody. Predicted molecular weight : 107-133 kDa (multiple isoforms) with a possible ~57 kDa isoform.
Flow cytometry testing of human HL60 cells with SEC31A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC31A antibody.

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NSJ-RQ7589-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein transport protein Sec31A is a protein that in humans is encoded by the SEC31A gene. The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Y101-K806) was used as the immunogen for the SEC31A antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O94979