NSJ Bioreagents

TTC7B Antibody / Tetratricopeptide repeat protein 7B

Product Code:
 
NSJ-RQ7598
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TTC7B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 10
Immunofluorescent staining of FFPE human ovarian cancer tissue with TTC7B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 10
IHC staining of FFPE human ovarian cancer tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human lung cancer tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human colorectal adenocarcinoma tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human breast cancer tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human liver cancer tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE rat brain tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 10
Western blot testing of 1) human HEL, 2) rat brain and 3) mouse brain tissue lysate with TTC7B antibody. Predicted molecular weight ~94 kDa.
10 / 10
Flow cytometry testing of human HepG2 cells with TTC7B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TTC7B antibody.
❮❯

Immunofluorescent staining of FFPE human ovarian cancer tissue with TTC7B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human ovarian cancer tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with TTC7B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HEL, 2) rat brain and 3) mouse brain tissue lysate with TTC7B antibody. Predicted molecular weight ~94 kDa.
Flow cytometry testing of human HepG2 cells with TTC7B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TTC7B antibody.

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CodeSizePrice
NSJ-RQ7598-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Component of a complex required to localize phosphatidylinositol 4-kinase(PI4K) to the plasma membrane. The complex acts as a regulator of phosphatidylinositol 4-phosphate(PtdIns(4)P) synthesis. In the complex, plays a central role in bridging PI4KA to EFR3B and FAM126A, via direct interactions
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E275-H730) was used as the immunogen for the TTC7B antibody.
Localisation:
Cytoplasm, cell membrane
Purity:
Antigen affinity purified
Uniprot #:
Q86TV6