NSJ Bioreagents

ATF6 Antibody / Activating transcription factor 6

Product Code:
 
NSJ-RQ7601
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ATF6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human SiHa cells with ATF6 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) 293T, 3) K562 and 4) MCF7 cell lysate with ATF6 antibody. Expected molecular weight: 75-90 kDa.
3 / 3
Flow cytometry testing of human SiHa cells with ATF6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATF6 antibody.
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Immunofluorescent staining of FFPE human SiHa cells with ATF6 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T, 3) K562 and 4) MCF7 cell lysate with ATF6 antibody. Expected molecular weight: 75-90 kDa.
Flow cytometry testing of human SiHa cells with ATF6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATF6 antibody.

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CodeSizePrice
NSJ-RQ7601-100ug100 ug£535.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ATF6, a member of the leucine zipper protein family, is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules. This gene is mapped to chromosome 1q23.3. ATF6 can constitutively induce the promoter of glucose-regulated protein (grp) genes through activation of the endoplasmic reticulum (ER) stress element (ERSE).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q79-Q670) was used as the immunogen for the ATF6 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P18850