NSJ Bioreagents

MFG-E8 Antibody / Lactadherin / Milk Fat Globule

Product Code:
 
NSJ-RQ7609
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MFG-E8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
IHC staining of FFPE human colorectal adenocarcinoma tissue with MFG-E8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 2
Western blot testing of 1) human milk (5ul), 2) human milk (5ul), 3) human milk (2.5ul) and 4) human milk (2.5ul) with Lactadherin antibody. Expected molecular weight: 30-66 kDa depending glycosylation level.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with MFG-E8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human milk (5ul), 2) human milk (5ul), 3) human milk (2.5ul) and 4) human milk (2.5ul) with Lactadherin antibody. Expected molecular weight: 30-66 kDa depending glycosylation level.

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CodeSizePrice
NSJ-RQ7609-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al.(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids FKVAYSLNGHEFDFIHDVNKKHKE from the human protein were used as the immunogen for the MFG-E8 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q08431