NSJ Bioreagents

Semaphorin-4F Antibody / SEMA4F

Product Code:
 
NSJ-RQ7620
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Semaphorin-4F antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 12
IHC staining of FFPE mouse brain tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 12
IHC staining of FFPE rat brain tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 12
IHC staining of FFPE human ovarian serous cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 12
IHC staining of FFPE human breast cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 12
IHC staining of FFPE human thyroid cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 12
IHC staining of FFPE human liver cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE human lung adenocarcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 12
IHC staining of FFPE human colon adenocarcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 12
IHC staining of FFPE human larynx squamous cell carcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 12
Immunofluorescent staining of FFPE human U-2 OS cells with Semaphorin-4F antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
11 / 12
Western blot testing of 1) human U-87 MG, 2) rat lung, 3) rat C6 and 4) mouse lung tissue lysate with Semaphorin-4F antibody. Predicted molecular weight ~66 kDa but may be observed at higher molecular weights due to glycosylation.
12 / 12
Flow cytometry testing of human Daudi cells with Semaphorin-4F antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Semaphorin-4F antibody.
❮❯

IHC staining of FFPE mouse brain tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with Semaphorin-4F antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human U-87 MG, 2) rat lung, 3) rat C6 and 4) mouse lung tissue lysate with Semaphorin-4F antibody. Predicted molecular weight ~66 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human Daudi cells with Semaphorin-4F antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Semaphorin-4F antibody.

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CodeSizePrice
NSJ-RQ7620-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Semaphorin-4F is a protein that in humans is encoded by the SEMA4F gene. This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R35-E574) was used as the immunogen for the Semaphorin-4F antibody.
Purity:
Antigen affinity purified
Uniprot #:
O95754