NSJ Bioreagents

Alpha-1-Antichymotrypsin Antibody / AACT / SERPINA3

Product Code:
 
NSJ-RQ7626
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Alpha-1-Antichymotrypsin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HepG2 cells with Alpha-1-Antichymotrypsin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alpha-1-Antichymotrypsin antibody.
2 / 2
Western blot testing of human 1) HepG2, 2) HCCT and 3) HUH-7 cell lysate with Alpha-1-Antichymotrypsin antibody. Expected molecular weight: 47-65 kDa, depending on level of glycosylation.
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Flow cytometry testing of human HepG2 cells with Alpha-1-Antichymotrypsin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alpha-1-Antichymotrypsin antibody.
Western blot testing of human 1) HepG2, 2) HCCT and 3) HUH-7 cell lysate with Alpha-1-Antichymotrypsin antibody. Expected molecular weight: 47-65 kDa, depending on level of glycosylation.

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NSJ-RQ7626-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Alpha 1-antichymotrypsin, also called serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 or GIG24 is an alpha globulin glycoprotein that is a member of the serpin superfamily. In humans, it is encoded by the SERPINA3 gene. This gene is mapped to 14q32.13. The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H24-A423) was used as the immunogen for the Alpha-1-Antichymotrypsin antibody.
Purity:
Antigen affinity purified
Uniprot #:
P01011