NSJ Bioreagents

SF3a60 Antibody / SAP 61 / Splicing factor 3A subunit 3

Product Code:
 
NSJ-RQ7634
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SF3a60 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 14
IHC staining of FFPE human ovarian serous cancer tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 14
IHC staining of FFPE human lung adenocarcinoma tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 14
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 14
IHC staining of FFPE human spleen tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 14
IHC staining of FFPE human prostate adenocarcinoma tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 14
IHC staining of FFPE human breast cancer tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 14
IHC staining of FFPE mouse brain tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 14
IHC staining of FFPE mouse kidney tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 14
IHC staining of FFPE rat brain tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 14
IHC staining of FFPE rat kidney tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 14
Immunofluorescent staining of FFPE human intestinal cancer tissue with SF3a60 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
12 / 14
Immunofluorescent staining of FFPE human PC-3 cells with SF3a60 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
13 / 14
Western blot testing of 1) human HeLa, 2) human A431, 3) human HL60, 4) rat liver, 5) rat C6, 6) mouse liver and 7) mouse NIH 3T3 cell lysate with SF3a60 antibody. Predicted molecular weight ~59 kDa.
14 / 14
Flow cytometry testing of human SiHa cells with SF3a60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SF3a60 antibody.
❮❯

IHC staining of FFPE human ovarian serous cancer tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human intestinal cancer tissue with SF3a60 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human PC-3 cells with SF3a60 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human A431, 3) human HL60, 4) rat liver, 5) rat C6, 6) mouse liver and 7) mouse NIH 3T3 cell lysate with SF3a60 antibody. Predicted molecular weight ~59 kDa.
Flow cytometry testing of human SiHa cells with SF3a60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SF3a60 antibody.

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NSJ-RQ7634-100ug100 ug£535.00
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Splicing factor 3A subunit 3 is a protein that in humans is encoded by the SF3A3 gene. This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E123-Q472) was used as the immunogen for the SF3a60 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q12874