NSJ Bioreagents

Ferroportin 1 Antibody / FPN / SLC40A1

Product Code:
 
NSJ-RQ7663
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Ferroportin 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
Immunofluorescent staining of FFPE human ovarian cancer tissue with Ferroportin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 8
IHC staining of FFPE human prostate adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human rectum adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human larynx squamous cell carcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE human liver cancer tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of 1) human plasma, 2) rat liver, 3) mouse spleen and 4) mouse liver tissue lysate with Ferroportin 1 antibody. Predicted molecular weight: ~62 kDa, but may be observed at higher molecular weights due to glycosylation.
8 / 8
Flow cytometry testing of human U-2 OS cells with Ferroportin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ferroportin 1 antibody.
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Immunofluorescent staining of FFPE human ovarian cancer tissue with Ferroportin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human prostate adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectum adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human plasma, 2) rat liver, 3) mouse spleen and 4) mouse liver tissue lysate with Ferroportin 1 antibody. Predicted molecular weight: ~62 kDa, but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human U-2 OS cells with Ferroportin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ferroportin 1 antibody.

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CodeSizePrice
NSJ-RQ7663-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ferroportin-1, also known as solute carrier family 40 member 1 (SLC40A1) or iron-regulated transporter 1 (IREG1), is a protein that in humans is encoded by the SLC40A1 gene, and is part of the Ferroportin (Fpn) Family.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-V571) was used as the immunogen for the Ferroportin 1 antibody.
Localisation:
Cell membrane, cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
Q9NP59