NSJ Bioreagents

SNF2H Antibody / Sucrose nonfermenting protein 2 / SMARCA5

Product Code:
 
NSJ-RQ7715
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SNF2H antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 13
Immunofluorescent staining of FFPE human A549 cells with SNF2H antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 13
IHC staining of FFPE human thyroid papillary carcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 13
IHC staining of FFPE human urothelial carcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 13
IHC staining of FFPE human placental tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 13
IHC staining of FFPE human colon adenocarcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 13
IHC staining of FFPE human spleen tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 13
IHC staining of FFPE human esophageal squamous carcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 13
IHC staining of FFPE human appendix adenocarcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 13
IHC staining of FFPE human breast cancer tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 13
IHC staining of FFPE human lung adenocarcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 13
IHC staining of FFPE human endometrioid adenocarcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 13
Western blot testing of human 1) HepG2 and 2) HeLa cell lysate with SNF2H antibody. Predicted molecular weight ~122 kDa.
13 / 13
Flow cytometry testing of human ThP-1 cells with SNF2H antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SNF2H antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with SNF2H antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human thyroid papillary carcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendix adenocarcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrioid adenocarcinoma tissue with SNF2H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HepG2 and 2) HeLa cell lysate with SNF2H antibody. Predicted molecular weight ~122 kDa.
Flow cytometry testing of human ThP-1 cells with SNF2H antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SNF2H antibody.

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NSJ-RQ7715-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 is a protein that in humans is encoded by the SMARCA5 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids A54-K735) was used as the immunogen for the SNF2H antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
O60264