NSJ Bioreagents

SMC2 Antibody / Structural maintenance of chromosomes protein 2

Product Code:
 
NSJ-RQ7719
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SMC2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with SMC2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human 293T, 2) human HeLa, 3) rat C6 and 4) mouse NIH 3T3 cell lysate with SMC2 antibody. Predicted molecular weight ~136 kDa.
3 / 3
Flow cytometry testing of human 293T cells with SMC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMC2 antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with SMC2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human HeLa, 3) rat C6 and 4) mouse NIH 3T3 cell lysate with SMC2 antibody. Predicted molecular weight ~136 kDa.
Flow cytometry testing of human 293T cells with SMC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMC2 antibody.

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CodeSizePrice
NSJ-RQ7719-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Structural maintenance of chromosomes protein 2 (SMC-2) also known as chromosome-associated protein E (CAP-E) is a protein that in humans is encoded by the SMC2 gene. Predicted to enable ATP binding activity; chromatin binding activity; and single-stranded DNA binding activity. Involved in mitotic chromosome condensation. Located in condensed chromosome; cytoplasm; and nuclear lumen. Part of condensin complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids L359-K1071) was used as the immunogen for the SMC2 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O95347