NSJ Bioreagents

SMC5 Antibody / Structural maintenance of chromosomes protein 5

Product Code:
 
NSJ-RQ7720
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SMC5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human HeLa cells with SMC5 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human MOLT4, 3) rat testis and 4) mouse NIH 3T3 cell lysate with SMC5 antibody. Predicted molecular weight ~129 kDa.
3 / 3
Flow cytometry testing of human THP-1 cells with SMC5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMC5 antibody.
❮❯

Immunofluorescent staining of FFPE human HeLa cells with SMC5 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human MOLT4, 3) rat testis and 4) mouse NIH 3T3 cell lysate with SMC5 antibody. Predicted molecular weight ~129 kDa.
Flow cytometry testing of human THP-1 cells with SMC5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMC5 antibody.

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CodeSizePrice
NSJ-RQ7720-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Structural maintenance of chromosomes protein 5 is a protein encoded by the SMC5 gene in human. Predicted to enable ATP binding activity. Involved in several processes, including DNA recombination; cellular senescence; and positive regulation of maintenance of mitotic sister chromatid cohesion. Located in cell junction; chromosome; and nuclear body. Part of Smc5-Smc6 complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q14-Q1089) was used as the immunogen for the SMC5 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q8IY18