NSJ Bioreagents

SMC6 Antibody / Structural maintenance of chromosomes protein 6

Product Code:
 
NSJ-RQ7721
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SMC6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
Immunofluorescent staining of FFPE human breast cancer tissue with SMC6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 5
Immunofluorescent staining of FFPE human A549 cells with SMC6 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
IHC staining of FFPE human breast cancer tissue with SMC6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human K562, 2) human HEL, 3) human A431, 4) human U-251 and 5) rat testis tissue lysate with SMC6 antibody. Predicted molecular weight ~126 kDa.
5 / 5
Flow cytometry testing of human 293T cells with SMC6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMC6 antibody.
❮❯

Immunofluorescent staining of FFPE human breast cancer tissue with SMC6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with SMC6 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer tissue with SMC6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human K562, 2) human HEL, 3) human A431, 4) human U-251 and 5) rat testis tissue lysate with SMC6 antibody. Predicted molecular weight ~126 kDa.
Flow cytometry testing of human 293T cells with SMC6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMC6 antibody.

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CodeSizePrice
NSJ-RQ7721-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Structural maintenance of chromosomes protein 6 is a protein that in humans is encoded by the SMC6 gene. Structural maintenance of chromosomes protein 6, also knowns SMC6L1, is a protein that in humans is encoded by the SMC6 gene. It is involved in the Alternative lengthening of telomeres cancer mechanism. The International Radiation Hybrid Mapping Consortium mapped the SMC6L1 gene to chromosome 2.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H499-D1018) was used as the immunogen for the SMC6 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q96SB8