NSJ Bioreagents

CBLIF Antibody / Cobalamin binding intrinsic factor / GIF

Product Code:
 
NSJ-RQ7732
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CBLIF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with CBLIF antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HGC-27 and 2) mouse stomach tissue lysate with CBLIF antibody. Predicted molecular weight ~45 kDa but may be observed at higher molecular weights due to glycosylation.
3 / 3
Flow cytometry testing of human U937 cells with CBLIF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBLIF antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with CBLIF antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HGC-27 and 2) mouse stomach tissue lysate with CBLIF antibody. Predicted molecular weight ~45 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human U937 cells with CBLIF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBLIF antibody.

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CodeSizePrice
NSJ-RQ7732-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E128-Y417) was used as the immunogen for the CBLIF antibody.
Purity:
Antigen affinity purified
Uniprot #:
P27352