NSJ Bioreagents

RASGRP1 Antibody / RAS guanyl-releasing protein 1

Product Code:
 
NSJ-RQ7733
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RASGRP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Flow cytometry testing of human U-2 OS cells with RASGRP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RASGRP1 antibody.
2 / 3
Flow cytometry testing of human MCF7 cells with RASGRP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RASGRP1 antibody.
3 / 3
Western blot testing of human 1) Jurkat, 2) 293T and 3) U-87 MG cell lysate with RASGRP1 antibody. Predicted molecular weight ~90 kDa with multiple smaller isoforms.
❮❯

Flow cytometry testing of human U-2 OS cells with RASGRP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RASGRP1 antibody.
Flow cytometry testing of human MCF7 cells with RASGRP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RASGRP1 antibody.
Western blot testing of human 1) Jurkat, 2) 293T and 3) U-87 MG cell lysate with RASGRP1 antibody. Predicted molecular weight ~90 kDa with multiple smaller isoforms.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ7733-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RAS guanyl-releasing protein 1 is a protein that in humans is encoded by the RASGRP1 gene. This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R8-Q678) was used as the immunogen for the RASGRP1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
O95267