NSJ Bioreagents

U2 snRNP A Antibody / SNRPA1

Product Code:
 
NSJ-RQ7735
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the U2 snRNP A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 15
Immunofluorescent staining of FFPE human lung cancer tissue with U2 snRNP A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 15
Immunofluorescent staining of FFPE human prostate cancer tissue with U2 snRNP A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 15
Immunofluorescent staining of FFPE mouse brain tissue with U2 snRNP A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
4 / 15
Immunofluorescent staining of FFPE rat brain tissue with U2 snRNP A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
5 / 15
Immunofluorescent staining of FFPE human Caco-2 cells with U2 snRNP A antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 15
IHC staining of FFPE human larynx squamous cell carcinoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 15
IHC staining of FFPE human lung adenocarcinoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 15
IHC staining of FFPE human glioblastoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 15
IHC staining of FFPE human placental tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 15
IHC staining of FFPE human prostate adenocarcinoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 15
IHC staining of FFPE human testicular seminoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 15
IHC staining of FFPE mouse brain tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
13 / 15
IHC staining of FFPE rat brain tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
14 / 15
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Jurkat, 4) human Raji, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with U2 snRNP A antibody. Predicted molecular weight ~28 kDa.
15 / 15
Flow cytometry testing of human MCF7 cells with U2 snRNP A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= U2 snRNP A antibody.
❮❯

Immunofluorescent staining of FFPE human lung cancer tissue with U2 snRNP A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human prostate cancer tissue with U2 snRNP A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE mouse brain tissue with U2 snRNP A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with U2 snRNP A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human Caco-2 cells with U2 snRNP A antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioblastoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular seminoma tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with U2 snRNP A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Jurkat, 4) human Raji, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with U2 snRNP A antibody. Predicted molecular weight ~28 kDa.
Flow cytometry testing of human MCF7 cells with U2 snRNP A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= U2 snRNP A antibody.

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CodeSizePrice
NSJ-RQ7735-100ug100 ug£535.00
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
U2 small nuclear ribonucleoprotein A' is a protein that in humans is encoded by the SNRPA1 gene. Enables RNA binding activity. Involved in mRNA splicing, via spliceosome and spermatogenesis. Located in nuclear speck. Part of U2-type catalytic step 2 spliceosome and U2-type precatalytic spliceosome. Implicated in connective tissue disease.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-E233) was used as the immunogen for the U2 snRNP A antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P09661