NSJ Bioreagents

Retinol Binding Protein 1 Antibody / RBP1

Product Code:
 
NSJ-RQ7744
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Retinol Binding Protein 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
Immunofluorescent staining of FFPE human U-2 OS cells with Retinol Binding Protein 1 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 8
IHC staining of FFPE human glioblastoma tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human liver cancer tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human rectum adenocarcinoma tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE mouse ovary tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE rat ovary tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of 1) human SH-SY5Y and 2) rat liver tissue lysate with Retinol Binding Protein 1 antibody. Predicted molecular weight ~16 kDa.
8 / 8
Flow cytometry testing of human U-2 OS cells with Retinol Binding Protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Retinol Binding Protein 1 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Retinol Binding Protein 1 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human glioblastoma tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectum adenocarcinoma tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse ovary tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat ovary tissue with Retinol Binding Protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human SH-SY5Y and 2) rat liver tissue lysate with Retinol Binding Protein 1 antibody. Predicted molecular weight ~16 kDa.
Flow cytometry testing of human U-2 OS cells with Retinol Binding Protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Retinol Binding Protein 1 antibody.

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CodeSizePrice
NSJ-RQ7744-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Retinol binding protein 1, cellular, also known as RBP1, is a protein that in humans is encoded by the RBP1 gene. This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K10-Q135) was used as the immunogen for the Retinol Binding Protein 1 antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
P09455