NSJ Bioreagents

Regulatory factor X 3 Antibody / RFX3

Product Code:
 
NSJ-RQ7762
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Regulatory factor X 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 6
Immunofluorescent staining of FFPE human U-2 OS cells with Regulatory factor X 3 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
Immunofluorescent staining of FFPE rat brain tissue with Regulatory factor X 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 6
IHC staining of FFPE mouse brain tissue with Regulatory factor X 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat brain tissue with Regulatory factor X 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) HEL and 2) SH-SY5Y cell lysate with Regulatory factor X 3 antibody. Predicted molecular weight ~84 kDa.
6 / 6
Flow cytometry testing of human RT4 cells with Regulatory factor X 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Regulatory factor X 3 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Regulatory factor X 3 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with Regulatory factor X 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE mouse brain tissue with Regulatory factor X 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Regulatory factor X 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEL and 2) SH-SY5Y cell lysate with Regulatory factor X 3 antibody. Predicted molecular weight ~84 kDa.
Flow cytometry testing of human RT4 cells with Regulatory factor X 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Regulatory factor X 3 antibody.

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CodeSizePrice
NSJ-RQ7762-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Transcription factor RFX3 is a protein that in humans is encoded by the RFX3 gene. This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q283-V749) was used as the immunogen for the Regulatory factor X 3 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P48380