NSJ Bioreagents

ArgRS Antibody / RARS1 / Arginyl-tRNA synthetase

Product Code:
 
NSJ-RQ7768
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ArgRS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 6
Immunofluorescent staining of FFPE human U-2 OS cells with ArgRS antibody (red), Alpha Tubulin mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC staining of FFPE human intestinal cancer tissue with ArgRS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse brain tissue with ArgRS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat brain tissue with ArgRS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human A431, 4) human 293T, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse NIH 3T3 cell lysate with ArgRS antibody. Predicted molecular weight ~75 kDa and ~67 kDa (two isoforms).
6 / 6
Flow cytometry testing of human MCF7 cells with ArgRS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ArgRS antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with ArgRS antibody (red), Alpha Tubulin mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human intestinal cancer tissue with ArgRS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with ArgRS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with ArgRS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human A431, 4) human 293T, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse NIH 3T3 cell lysate with ArgRS antibody. Predicted molecular weight ~75 kDa and ~67 kDa (two isoforms).
Flow cytometry testing of human MCF7 cells with ArgRS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ArgRS antibody.

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CodeSizePrice
NSJ-RQ7768-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H165-M425) was used as the immunogen for the ArgRS antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P54136