NSJ Bioreagents

EZH1 Antibody / Enhancer of zeste homolog 1

Product Code:
 
NSJ-RQ7773
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the EZH1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Western blot testing of 1) rat testis, 2) rat C6, 3) mouse testis and 4) mouse NIH 3T3 cell lysate with EZH1 antibody. Predicted molecular weight: 69-86 kDa (multiple isoforms).
2 / 4
Western blot testing of human 1) HepG2, 2) HL60, 3) 293T, 4) Daudi, 5) HaCaT, 6) HeLa and 7) MOLT4 cell lysate with EZH1 antibody. Predicted molecular weight: 69-86 kDa (multiple isoforms).
3 / 4
Immunofluorescent staining of FFPE human HeLa cells with GNB1 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 4
Flow cytometry testing of human RT4 cells with EZH1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EZH1 antibody.
❮❯

Western blot testing of 1) rat testis, 2) rat C6, 3) mouse testis and 4) mouse NIH 3T3 cell lysate with EZH1 antibody. Predicted molecular weight: 69-86 kDa (multiple isoforms).
Western blot testing of human 1) HepG2, 2) HL60, 3) 293T, 4) Daudi, 5) HaCaT, 6) HeLa and 7) MOLT4 cell lysate with EZH1 antibody. Predicted molecular weight: 69-86 kDa (multiple isoforms).
Immunofluorescent staining of FFPE human HeLa cells with GNB1 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human RT4 cells with EZH1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EZH1 antibody.

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CodeSizePrice
NSJ-RQ7773-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Histone-lysine N-methyltransferase EZH1 is an enzyme that in humans is encoded by the EZH1 gene. EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R27-E485) was used as the immunogen for the EZH1 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q92800