NSJ Bioreagents

RBFA Antibody / Ribosome-binding factor A / C18orf22

Product Code:
 
NSJ-RQ7789
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RBFA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 9
Immunofluorescent staining of FFPE human U-2 OS cells with RBFA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 9
IHC staining of FFPE human undifferentiated carcinoma of pancreas tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human breast cancer tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human appendix adenocarcinoma tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human lung squamous cell carcinoma tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human esophageal squamous carcinoma tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
IHC staining of FFPE human liver cancer tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 9
Western blot testing of human 1) Daudi, 2) MOLT4 and 3) HepG2 cell lysate with RBFA antibody. Predicted molecular weight ~38 kDa.
9 / 9
Flow cytometry testing of human MCF7 cells with RBFA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBFA antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with RBFA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human undifferentiated carcinoma of pancreas tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendix adenocarcinoma tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung squamous cell carcinoma tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with RBFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Daudi, 2) MOLT4 and 3) HepG2 cell lysate with RBFA antibody. Predicted molecular weight ~38 kDa.
Flow cytometry testing of human MCF7 cells with RBFA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBFA antibody.

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CodeSizePrice
NSJ-RQ7789-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Predicted to be involved in rRNA processing. Predicted to be located in mitochondrion.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids L15-D319) was used as the immunogen for the RBFA antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q8N0V3