NSJ Bioreagents

RBM25 Antibody / RNA-binding protein 25

Product Code:
 
NSJ-RQ7808
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RBM25 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human esophageal squamous carcinoma tissue with RBM25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE mouse brain tissue with RBM25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat brain tissue with RBM25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) HeLa, 2) Jurkat, 3) 293T and 4) Daudi cell lysate with RBM25 antibody. Predicted molecular weight ~100 kDa but commonly observed at ~120 kDa.
5 / 5
Flow cytometry testing of human Jurkat cells with RBM25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBM25 antibody.
❮❯

IHC staining of FFPE human esophageal squamous carcinoma tissue with RBM25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with RBM25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with RBM25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) 293T and 4) Daudi cell lysate with RBM25 antibody. Predicted molecular weight ~100 kDa but commonly observed at ~120 kDa.
Flow cytometry testing of human Jurkat cells with RBM25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBM25 antibody.

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CodeSizePrice
NSJ-RQ7808-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RNA-binding protein 25, also called Arg/Glu/Asp-rich protein of 120 kDa (RED120), is a protein that in humans is encoded by the RBM25 gene. Enables mRNA binding activity. Involved in regulation of alternative mRNA splicing, via spliceosome and regulation of apoptotic process. Located in nuclear speck.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D100-E820) was used as the immunogen for the RBM25 antibody.
Localisation:
Cytoplasmic, nuclear specks
Purity:
Antigen affinity purified
Uniprot #:
P49756