NSJ Bioreagents

Cd39 Antibody / Entpd1

Product Code:
 
NSJ-RQ7811
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Cd39 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
IHC staining of FFPE rat spleen tissue with Cd39 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) rat spleen, 2) mouse spleen, 3) rat C6, 4) rat brain, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with Cd39 antibody. Expected molecular weight: 34-100 kDa depending on isoform and level of glycosylation.
3 / 3
Flow cytometry testing of mouse ANA-1 cells with Cd39 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd39 antibody.
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IHC staining of FFPE rat spleen tissue with Cd39 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat spleen, 2) mouse spleen, 3) rat C6, 4) rat brain, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with Cd39 antibody. Expected molecular weight: 34-100 kDa depending on isoform and level of glycosylation.
Flow cytometry testing of mouse ANA-1 cells with Cd39 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd39 antibody.

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CodeSizePrice
NSJ-RQ7811-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ectonucleoside triphosphate diphosphohydrolase-1 (gene: ENTPD1; protein: NTPDase1) also known as CD39 (Cluster of Differentiation 39), is a typical cell surface enzyme with a catalytic site on the extracellular face. Enables adenosine-diphosphatase activity and nucleoside-triphosphatase activity. Involved in ADP catabolic process. Acts upstream of or within G protein-coupled receptor signaling pathway; platelet activation; and purine ribonucleoside diphosphate catabolic process. Located in basement membrane. Is active in external side of plasma membrane. Is expressed in several structures, including alimentary system; genitourinary system; heart; nervous system; and respiratory system. Human ortholog(s) of this gene implicated in hereditary spastic paraplegia 64. Orthologous to human ENTPD1 (ectonucleoside triphosphate diphosphohydrolase 1).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q39-S475) was used as the immunogen for the Cd39 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P55772