NSJ Bioreagents

Pirh2 Antibody / RCHY1

Product Code:
 
NSJ-RQ7813
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Pirh2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HepG2 cells with Pirh2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pirh2 antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Daudi, 4) human 293T, 5) rat liver, 6) rat PC-12 and 7) mouse liver tissue lysate with Pirh2 antibody. Predicted molecular weight: 21-30 kDa (multiple isoforms) with an additional possbile ~8 kDa isoform.
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Flow cytometry testing of human HepG2 cells with Pirh2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pirh2 antibody.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Daudi, 4) human 293T, 5) rat liver, 6) rat PC-12 and 7) mouse liver tissue lysate with Pirh2 antibody. Predicted molecular weight: 21-30 kDa (multiple isoforms) with an additional possbile ~8 kDa isoform.

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NSJ-RQ7813-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RING finger and CHY zinc finger domain-containing protein 1, also called E3 ubiquitin-protein ligase Pirh2, is a protein that in humans is encoded by the RCHY1 gene. The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q17-Q250) was used as the immunogen for the Pirh2 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q96PM5