NSJ Bioreagents

ATG12 Antibody

Product Code:
 
NSJ-RQ7816
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ATG12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human RT4 cells with ATG12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG12 antibody.
2 / 2
Western blot testing of human 1) PC-3, 2) COLO-320, 3) HeLa and 4) HepG2 cell lysate with ATG12 antibody. Expected molecular weight: ~15 kDa (ATG12) and 50-60 kDa (ATG12-ATG5 complex).
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Flow cytometry testing of human RT4 cells with ATG12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG12 antibody.
Western blot testing of human 1) PC-3, 2) COLO-320, 3) HeLa and 4) HepG2 cell lysate with ATG12 antibody. Expected molecular weight: ~15 kDa (ATG12) and 50-60 kDa (ATG12-ATG5 complex).

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CodeSizePrice
NSJ-RQ7816-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Autophagy related 12 is a protein that in humans is encoded by the ATG12 gene. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M68-Q99) was used as the immunogen for the ATG12 antibody.
Purity:
Antigen affinity purified
Uniprot #:
O94817