NSJ Bioreagents

RFC2 Antibody / Replication factor C subunit 2

Product Code:
 
NSJ-RQ7817
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RFC2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human PC-3 cells with RFC2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human Raji, 4) human U-251, 5) rat C6, 6) rat PC-12, 7) mouse Neuro-2a and 8) mouse SP2/0 cell lysate with RFC2 antibody. Predicted molecular weight ~39 kDa.
3 / 3
Flow cytometry testing of human U-251 cells with RFC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RFC2 antibody.
❮❯

Immunofluorescent staining of FFPE human PC-3 cells with RFC2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human Raji, 4) human U-251, 5) rat C6, 6) rat PC-12, 7) mouse Neuro-2a and 8) mouse SP2/0 cell lysate with RFC2 antibody. Predicted molecular weight ~39 kDa.
Flow cytometry testing of human U-251 cells with RFC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RFC2 antibody.

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NSJ-RQ7817-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Replication factor C subunit 2, also called Activator 1 40 kDa subunit (A1 40 kDa subunit) and Replication factor C 40 kDa subunit (RF-C 40 kDa subunit or RFC40), is a protein that in humans is encoded by the RFC2 gene. This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E40-K313) was used as the immunogen for the RFC2 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P35250