NSJ Bioreagents

RFC3 Antibody / Replication factor C subunit 3

Product Code:
 
NSJ-RQ7818
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RFC3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human PC-3 cells with RFC3 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human Daudi, 4) rat liver and 5) mouse liver tissue lysate with RFC3 antibody. Predicted molecular weight: 38-41 kDa.
3 / 3
Flow cytometry testing of human U-251 cells with RFC3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RFC3 antibody.
❮❯

Immunofluorescent staining of FFPE human PC-3 cells with RFC3 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human Daudi, 4) rat liver and 5) mouse liver tissue lysate with RFC3 antibody. Predicted molecular weight: 38-41 kDa.
Flow cytometry testing of human U-251 cells with RFC3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RFC3 antibody.

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CodeSizePrice
NSJ-RQ7818-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Replication factor C subunit 3, also called Activator 1 38 kDa subunit (A1 38 kDa subunit) and Replication factor C 38 kDa subunit (RF-C 38 kDa subunit or RFC38), is a protein that in humans is encoded by the RFC3 gene. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D6-E333) was used as the immunogen for the RFC3 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P40938