NSJ Bioreagents

Sphingosine kinase 1 Antibody / SPHK1

Product Code:
 
NSJ-RQ7823
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Sphingosine kinase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HepG2 cells with Sphingosine kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sphingosine kinase 1 antibody.
2 / 2
Western blot testing of human 1) HeLa, 2) HepG2 and 3) MCF7 cell lysate with Sphingosine kinase 1 antibody. Predicted molecular weight: ~43/51/44 kDa (isoforms 1/2/3).
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Flow cytometry testing of human HepG2 cells with Sphingosine kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sphingosine kinase 1 antibody.
Western blot testing of human 1) HeLa, 2) HepG2 and 3) MCF7 cell lysate with Sphingosine kinase 1 antibody. Predicted molecular weight: ~43/51/44 kDa (isoforms 1/2/3).

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CodeSizePrice
NSJ-RQ7823-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SPHK1 (Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E55-N357) was used as the immunogen for the Sphingosine kinase 1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9NYA1