NSJ Bioreagents

TMEM50B Antibody / Transmembrane protein 50B / C21orf4

Product Code:
 
NSJ-RQ7826
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TMEM50B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human MCF7 cells with TMEM50B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human T-47D, 2) human HEL, 3) human ThP-1, 4) rat testis, 5) rat lung, 6) mouse testis and 7) mouse lung tissue lysate with TMEM50B antibody. Predicted molecular weight ~18 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with TMEM50B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM50B antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with TMEM50B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human T-47D, 2) human HEL, 3) human ThP-1, 4) rat testis, 5) rat lung, 6) mouse testis and 7) mouse lung tissue lysate with TMEM50B antibody. Predicted molecular weight ~18 kDa.
Flow cytometry testing of human U-87 MG cells with TMEM50B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM50B antibody.

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CodeSizePrice
NSJ-RQ7826-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Predicted to be involved in late endosome to vacuole transport via multivesicular body sorting pathway. Located in endoplasmic reticulum.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids GFLDNFRWPECECIDWSERR from the human protein were used as the immunogen for the TMEM50B antibody.
Localisation:
Nucleoli fibrillar center, ER
Purity:
Antigen affinity purified
Uniprot #:
P56557