NSJ Bioreagents

Girdin Antibody / CCDC88A / GIV

Product Code:
 
NSJ-RQ7828
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Girdin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HepG2 cells with Girdin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Girdin antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human SiHa, 3) rat brain and 4) rat RH-35 cell lysate with Girdin antibody. Predicted molecular weight ~216 kDa.
❮❯

Flow cytometry testing of human HepG2 cells with Girdin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Girdin antibody.
Western blot testing of 1) human HeLa, 2) human SiHa, 3) rat brain and 4) rat RH-35 cell lysate with Girdin antibody. Predicted molecular weight ~216 kDa.

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CodeSizePrice
NSJ-RQ7828-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Girdin, also called G alpha-interacting vesicle-associated protein (GIV), is a protein that in humans is encoded by the CCDC88A gene. This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K1231-D1836) was used as the immunogen for the Girdin antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q3V6T2