NSJ Bioreagents

Myeloperoxidase Antibody / MPO

Product Code:
 
NSJ-RQ7843
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Myeloperoxidase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HL60 cells with Myeloperoxidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Myeloperoxidase antibody.
2 / 2
Western blot testing of human HL60 cell lysate with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).

Flow cytometry testing of human HL60 cells with Myeloperoxidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Myeloperoxidase antibody.
Western blot testing of human HL60 cell lysate with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).

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CodeSizePrice
NSJ-RQ7843-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697(A49-S745) amino acids with the molecular mass of 80KDa.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K556-I697) was used as the immunogen for the Myeloperoxidase antibody.
Purity:
Antigen affinity purified
Uniprot #:
P05164