NSJ Bioreagents

HBBM Antibody / Heparin-binding brain mitogen / Pleiotrophin

Product Code:
 
NSJ-RQ7844
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the HBBM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human glioma tissue with HBBM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE mouse brain tissue with HBBM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat brain tissue with HBBM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) huma U-251, 2) rat brain, 3) rat C6 and 4) mouse brain tissue lysate with HBBM antibody. Predicted molecular weight ~19 kDa.
5 / 5
Flow cytometry testing of human U-2 OS cells with HBBM antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HBBM antibody.
❮❯

IHC staining of FFPE human glioma tissue with HBBM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with HBBM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with HBBM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) huma U-251, 2) rat brain, 3) rat C6 and 4) mouse brain tissue lysate with HBBM antibody. Predicted molecular weight ~19 kDa.
Flow cytometry testing of human U-2 OS cells with HBBM antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HBBM antibody.

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CodeSizePrice
NSJ-RQ7844-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Pleiotrophin (PTN), also known as heparin-binding brain mitogen (HBBM), heparin-binding growth factor 8 (HBGF-8), neurite growth-promoting factor 1 (NEGF1), heparin affinity regulatory peptide (HARP), heparin binding growth associated molecule (HB-GAM) and Osteoblast-specific factor 1 (OSF-1), is a protein that in humans is encoded by the PTN gene. PTN is the first member of a family of developmentally regulated cytokines. The PTN gene is mapped to 7q33-q34. A mutant PTN that contained only the first N-terminal 40 amino acids was a dominant negative. Pleiotrophin is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. Pleiotrophin binds to cell-surface nucleolin as a low affinity receptor. This binding can inhibit HIV infection.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D61-K148) was used as the immunogen for the HBBM antibody.
Purity:
Antigen affinity purified
Uniprot #:
P21246