NSJ Bioreagents

RIMS-binding protein 2 Antibody / RIMBP2

Product Code:
 
NSJ-RQ7851
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RIMS-binding protein 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 6
Immunofluorescent staining of FFPE mouse brain tissue with RIMS-binding protein 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 6
Immunofluorescent staining of FFPE rat brain tissue with RIMS-binding protein 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 6
IHC staining of FFPE mouse brain tissue with RIMS-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat brain tissue with RIMS-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human SH-SY5Y, 2) rat brain and 3) mouse brain tissue lysate with RIMS-binding protein 2 antibody. Predicted molecular weight ~116 kDa.
6 / 6
Flow cytometry testing of human U-2 OS cells with RIMS-binding protein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIMS-binding protein 2 antibody.

Immunofluorescent staining of FFPE mouse brain tissue with RIMS-binding protein 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with RIMS-binding protein 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE mouse brain tissue with RIMS-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with RIMS-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human SH-SY5Y, 2) rat brain and 3) mouse brain tissue lysate with RIMS-binding protein 2 antibody. Predicted molecular weight ~116 kDa.
Flow cytometry testing of human U-2 OS cells with RIMS-binding protein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIMS-binding protein 2 antibody.

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CodeSizePrice
NSJ-RQ7851-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RIMS binding protein 2 is a protein that in humans is encoded by the RIMBP2 gene. Predicted to be involved in neuromuscular synaptic transmission. Predicted to be located in plasma membrane and synapse. Predicted to be active in presynaptic active zone cytoplasmic component.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K351-H1049) was used as the immunogen for the RIMS-binding protein 2 antibody.
Purity:
Antigen affinity purified
Uniprot #:
O15034