NSJ Bioreagents

NNP1 Antibody / Novel nuclear protein 1 / RRP1

Product Code:
 
NSJ-RQ7866
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NNP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
Immunofluorescent staining of FFPE human U-87 MG cells with NPP1 antibody (red), Beta Tubulin mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human placental tissue with NNP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human colon adenocarcinoma tissue with NNP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) HeLa and 2) 293T cell lysate with NNP1 antibody. Predicted molecular weight ~53 kDa.
5 / 5
Flow cytometry testing of human HeLa cells with NNP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NNP1 antibody.
❮❯

Immunofluorescent staining of FFPE human U-87 MG cells with NPP1 antibody (red), Beta Tubulin mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placental tissue with NNP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma tissue with NNP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa and 2) 293T cell lysate with NNP1 antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human HeLa cells with NNP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NNP1 antibody.

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CodeSizePrice
NSJ-RQ7866-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ribosomal RNA processing protein 1 homolog A, also called Novel nuclear protein 1 (NNP1), is a protein that in humans is encoded by the RRP1 gene. The protein encoded by this gene is the putative homolog of the yeast ribosomal RNA processing protein RRP1. The encoded protein is involved in the late stages of nucleologenesis at the end of mitosis, and may be required for the generation of 28S rRNA.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q22-D237) was used as the immunogen for the NNP1 antibody.
Localisation:
Nucleus, Nucleolus
Purity:
Antigen affinity purified
Uniprot #:
P56182