NSJ Bioreagents

Ribophorin II Antibody / RPN2

Product Code:
 
NSJ-RQ7872
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Ribophorin II antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-87 MG cells with Ribophorin II antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) ThP-1, 2) HepG2 and 3) T-47D cell lysate with Ribophorin II antibody. Predicted molecular weight ~69 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with Ribophorin II antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribophorin II antibody.
❮❯

Immunofluorescent staining of FFPE human U-87 MG cells with Ribophorin II antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) ThP-1, 2) HepG2 and 3) T-47D cell lysate with Ribophorin II antibody. Predicted molecular weight ~69 kDa.
Flow cytometry testing of human HL60 cells with Ribophorin II antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribophorin II antibody.

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CodeSizePrice
NSJ-RQ7872-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 2, also called Ribophorin II, is an enzyme that in humans is encoded by the RPN2 gene. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q64-Q174) was used as the immunogen for the Ribophorin II antibody.
Localisation:
Cytoplasm (ER)
Purity:
Antigen affinity purified
Uniprot #:
P04844