NSJ Bioreagents

RTCB Antibody / C22orf28

Product Code:
 
NSJ-RQ7879
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RTCB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with RTCB antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HL60 and 4) 293T cell lysate with RTCB antibody. Predicted molecular weight ~55 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with RTCB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTCB antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with RTCB antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HL60 and 4) 293T cell lysate with RTCB antibody. Predicted molecular weight ~55 kDa.
Flow cytometry testing of human HepG2 cells with RTCB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTCB antibody.

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CodeSizePrice
NSJ-RQ7879-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RNA 2',3'-cyclic phosphate and 5'-OH ligase is a protein that in humans is encoded by the RTCB gene. Enables RNA ligase (ATP) activity and vinculin binding activity. Involved in tRNA splicing, via endonucleolytic cleavage and ligation. Located in cytosol and nucleoplasm. Part of tRNA-splicing ligase complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H227-G505) was used as the immunogen for the RTCB antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9Y3I0