NSJ Bioreagents

OGDH Antibody / 2-oxoglutarate dehydrogenase

Product Code:
 
NSJ-RQ7889
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the OGDH antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human Daudi cells with OGDH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OGDH antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Daudi, 4) rat lung, 5) rat heart, 6) mouse lung and 7) mouse heart tissue lysate with OGDH antibody. Predicted molecular weight ~116 kDa.
❮❯

Flow cytometry testing of human Daudi cells with OGDH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OGDH antibody.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Daudi, 4) rat lung, 5) rat heart, 6) mouse lung and 7) mouse heart tissue lysate with OGDH antibody. Predicted molecular weight ~116 kDa.

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CodeSizePrice
NSJ-RQ7889-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Alpha-ketoglutarate dehydrogenase also known as 2-oxoglutarate dehydrogenase E1 component, mitochondrial is an enzyme that in humans is encoded by the OGDH gene. This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-S1023) was used as the immunogen for the OGDH antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q02218