NSJ Bioreagents

RPS17 Antibody / 40S ribosomal protein S17

Product Code:
 
NSJ-RQ7890
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RPS17 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human PC-3 cells with RPS17 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human Thp-1, 3) human 293T, 4) human placenta, 5) rat ovary, 6) rat RH35 and 7) mouse NIH 3T3 cell lysate with RPS17 antibody. Predicted molecular weight ~16 kDa.
3 / 3
Flow cytometry testing of human A431 cells with RPS17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPS17 antibody.
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Immunofluorescent staining of FFPE human PC-3 cells with RPS17 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Thp-1, 3) human 293T, 4) human placenta, 5) rat ovary, 6) rat RH35 and 7) mouse NIH 3T3 cell lysate with RPS17 antibody. Predicted molecular weight ~16 kDa.
Flow cytometry testing of human A431 cells with RPS17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPS17 antibody.

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NSJ-RQ7890-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
40S ribosomal protein S17 is a protein that in humans is encoded by the RPS17 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-V135) was used as the immunogen for the RPS17 antibody.
Localisation:
Cytoplasm, nucleus, nucleolus
Purity:
Antigen affinity purified
Uniprot #:
P08708