NSJ Bioreagents

TSEN54 Antibody / tRNA-splicing endonuclease subunit Sen54

Product Code:
 
NSJ-RQ7897
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TSEN54 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human PC-3 cells with TSEN54 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human colorectal adenocarcinoma tissue with TSEN54 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) MCF7, 2) 293T, 3) Jurkat and 4) MOLT-4 cell lysate with TSEN54 antibody. Predicted molecular weight ~59 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with TSEN54 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TSEN54 antibody.

Immunofluorescent staining of FFPE human PC-3 cells with TSEN54 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human colorectal adenocarcinoma tissue with TSEN54 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) MCF7, 2) 293T, 3) Jurkat and 4) MOLT-4 cell lysate with TSEN54 antibody. Predicted molecular weight ~59 kDa.
Flow cytometry testing of human HeLa cells with TSEN54 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TSEN54 antibody.

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CodeSizePrice
NSJ-RQ7897-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene encodes a subunit of the tRNA splicing endonuclease complex, which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D147-H507) was used as the immunogen for the TSEN54 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q7Z6J9