NSJ Bioreagents

Dynamin-like 120 kDa protein Antibody / OPA1

Product Code:
 
NSJ-RQ7898
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Dynamin-like 120 kDa protein antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with Dynamin-like 120 kDa protein antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human Ramos, 2) human HeLa, 3) human PANC-1, 4) human MCF7, 5) rat brain, 6) mouse eye and 7) mouse brain tissue lysate with Dynamin-like 120 kDa protein antibody. Predicted molecular weight: 111-120 kDa with multiple smaller isoforms from 81-95 kDa.
3 / 3
Flow cytometry testing of human Daudi cells with Dynamin-like 120 kDa protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dynamin-like 120 kDa protein antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Dynamin-like 120 kDa protein antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Ramos, 2) human HeLa, 3) human PANC-1, 4) human MCF7, 5) rat brain, 6) mouse eye and 7) mouse brain tissue lysate with Dynamin-like 120 kDa protein antibody. Predicted molecular weight: 111-120 kDa with multiple smaller isoforms from 81-95 kDa.
Flow cytometry testing of human Daudi cells with Dynamin-like 120 kDa protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dynamin-like 120 kDa protein antibody.

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NSJ-RQ7898-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Dynamin-like 120 kDa protein, mitochondrial is a protein that in humans is encoded by the OPA1 gene. It is mapped to 3q29. This protein regulates mitochondrial fusion and cristae structure in the inner mitochondrial membrane (IMM) and contributes to ATP synthesis and apoptosis. This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D296-Q562) was used as the immunogen for the Dynamin-like 120 kDa protein antibody.
Localisation:
Cytoplasmic, membrane
Purity:
Antigen affinity purified
Uniprot #:
O60313