NSJ Bioreagents

RTF1 Antibody / RNA polymerase-ass°Ciated protein RTF1 homolog

Product Code:
 
NSJ-RQ7901
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RTF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE mouse brain tissue with RTF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat brain tissue with RTF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human Jurkat, 2) human SW620, 3) human MCF7 and 4) monkey COS-7 cell lysate with RTF1 antibody. Predicted molecular weight ~80 kDa, commonly observed at 80-100 kDa.
4 / 4
Flow cytometry testing of human Daudi cells with RTF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTF1 antibody.
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IHC staining of FFPE mouse brain tissue with RTF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with RTF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) human SW620, 3) human MCF7 and 4) monkey COS-7 cell lysate with RTF1 antibody. Predicted molecular weight ~80 kDa, commonly observed at 80-100 kDa.
Flow cytometry testing of human Daudi cells with RTF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTF1 antibody.

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CodeSizePrice
NSJ-RQ7901-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) is a protein that in humans is encoded by the RTF1 gene. This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling, based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids S77-K705) was used as the immunogen for the RTF1 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q92541