NSJ Bioreagents

SSBP1 Antibody / Single-stranded DNA-binding protein

Product Code:
 
NSJ-RQ7903
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SSBP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 9
Immunofluorescent staining of FFPE human ovarian cancer tissue with SSBP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 9
Immunofluorescent staining of FFPE human colon cancer tissue with SSBP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 9
Immunofluorescent staining of FFPE human U-2 OS cells with SSBP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 9
IHC staining of FFPE human lung adenocarcinoma tissue with SSBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human colon adenocarcinoma tissue with SSBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human endometrioid adenocarcinoma tissue with SSBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
IHC staining of FFPE human liver cancer tissue with SSBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 9
Western blot testing of human 1) HeLa, 2) 293T, 3) MOLT-4, 4) K562, 5) A549 and 6) A375 cell lysate with SSBP1 antibody. Predicted molecular weight ~17 kDa.
9 / 9
Flow cytometry testing of human HL60 cells with SSBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SSBP1 antibody.
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Immunofluorescent staining of FFPE human ovarian cancer tissue with SSBP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human colon cancer tissue with SSBP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with SSBP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung adenocarcinoma tissue with SSBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma tissue with SSBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrioid adenocarcinoma tissue with SSBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with SSBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) 293T, 3) MOLT-4, 4) K562, 5) A549 and 6) A375 cell lysate with SSBP1 antibody. Predicted molecular weight ~17 kDa.
Flow cytometry testing of human HL60 cells with SSBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SSBP1 antibody.

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CodeSizePrice
NSJ-RQ7903-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Single-stranded DNA-binding protein, mitochondrial is a protein that in humans is encoded by the SSBP1 gene. SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-E148) was used as the immunogen for the SSBP1 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q04837